ASPECTS OF CRYOPRESERVATION OF Clarias gariepinus OBTAINED FROM THE LOWER RIVER NIGER
TABLE OF CONTENTS
Title page i
Certification ii
Dedication iii
Acknowledgment iv
Table of contents v
Abstract vi
CHAPTER ONE
1.1 Introduction 1
1.2 Justification 4
1.3 Aims and Objectives 4
CHAPTER TWO
2.1 Literature Review 5
2.2 Sperm Mophology 6
2.3 Sperm Quality 7
2.4 Sperm Motility 7
2.5 Membrane Integrity 8
2.6 Extenders 9
2.7 Cryoprotectants 11
2.8 Effect of cryopreservation on sperm motility and fertility 12
2.9 Computer Assisted Sperm Analysis of velocity 14
CHAPTER THREE
3.0 Materials and Methods 17
3.1 Description of the study area 17
3.2 Collection and Management of fish 17
3.3 Methodology 18
3.3.1 Preparation of buffer 18
3.3.2 Preparation of Extender 18
3.3.3 Precurement and dilution of milt 19
3.3.4 Equilibration and Storage 20
3.3.5 Determination of Post-thawed parameters 21
CHAPTER FOUR
4.1 Morphemetric Characteristics of Experimental Fish 22
4.2 Sperm Characteristics 23
4.3 Sperm Motility/mortality 24
4.4 % Mean Motility 25
CHAPTER FIVE
5.1 Discussion 28
5.2 Summary and Conclusion 29
References 30
Appendix 35
ABSTRACT
To service the growing demand of insufficient supply of (Clarias gariepinus) brookstock for aquaculture in Nigeria, Conserve valuable genetic resources and with the intent of obtaining a semen preservation protocol that can serve as a means of making fingerlings available to fish farmers all year round, this study assessed cryopreservation (Clarias gariepinus) of three specimen were discested and milt preserved. Result showed that there was significance difference between fresh semen and control. Cryopreservation of sperm, remains essentially a research activity that is just beginning to have commercial application. It is hope their efforts will be geared towards standardization of research result to enhance beneficial repeatability by interested fish farmers
CHAPTER ONE
1.1 INTRODUCTION
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1.2 JUSTIFICATION
As fish farming expands and harvesting of wild stocks becomes more intense, there is a growing need for techniques of gamete storage to facilitate replacement of extinct fishes.
Cryopreservation of sperm is now a widely used method for long term storage of fish milt. It is effective tool for conserving genetic materials in threatened and endangered species, which can also be used for genetic improvement through selecting breeding programme.
1.3 AIMS AND OBJECTIVE
1. To evaluate the sperm characteristics of C. gariepinus.
2. To determine efficacy of some extenders in the preservation of milt of C. gariepinus.
CHAPTER TWO
