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THE EFFECT OF CISSAMPELOS MUCRONATA ON SODIUM/POTASSIUM ATPase In THE KIDNEY AND SERUM OF WISTAR RAT

3.2 METHODS

A total of 24 adult albino wistar rats (100g-250g) was procured from the animal breeding unit of Department Of Pharmacology and Therapeutics, Delta State University, Abraka, Nigeria was used for the study. The rats were housed in a well-ventilated room at a temperature of 25ºC - 28ºC and maintained under 12 hours light/dark cycle. The rats were fed pellets diet and water ad libitum. The animals were acclimatized to laboratory conditions for seven (7) days before commencement of the experiment. The study was performed in accordance with the Institutional Animal Ethical Committee (IAEC) of CPCSEA (Committee for the purpose of Control and Supervision of Experiments on Animals) of the Faculty of Basic Medical Sciences, Delta State University, Abraka.

3.3 PLANT COLLECTION AND IDENTIFICATION Cissampelosmucronata fresh leaves were collected beside Ethiope River behind Ethiope Hall at site 11, Abraka campus of Delta State University. The leaves were botanically identified and authenticated by a taxonomist, Department of Botany, Delta State University, Abraka.

3.4 PREPARATION OF PLANT EXTRACT

The leaves of Cissampelosmucronatathat was collected were carefully washed and dried in the sun for three (3) days. The leaves were grounded into powder using an electrical blender. The powder (100g) was macerated with ethanol for 48 hours with occasional shaking. The extract was concentrated by using rotary evaporator, the ethanol macerated extract was evaporated with rotary evaporator for about 24 hours. The extract was preserved in a desiccator till further use.

3.5 PREPARATION OF PLANT EXTRACT The leaves of Cissampelosmucronata were carefully washed and dried in sun for three days. The leaves were grounded into powder using an electrical blender. The powder (100g) was macerated with ethanol for 48 hours with occasional shaking. The extract was concentrated by using rotary evaporator. The extract was preserved in a dessicator till further use.

3.6 PREPARATION OF STOCK SOLUTION 3g extract of Cissampelosmucronata were dissolved in 300ml of distilled water. This gave a stock solution of 100mg/100ml. The stock solution were stored in the refrigerator before use.

3.6 EXPERIMENTAL DESIGN In the experiment, a total of 24 rats were used which was divided into four (4) groups of six (6) rats in each. They were administered various doses of ethanolic extract of the leaves of Cissampelosmucronata for three weeks at a concentration of 0.01g/ml/kg body weight.

3.7 EXPERIMENTAL DESIGN In the experimental, a total of 24 rats were used which was divided into four groups of six rats in each. They were administered with varying doses of ethanolic extract of the leaves of Cissampelosmucronata for two weeks at a concentration of 0.01g/ml/kg body weight. Group 1 received water ad libitum Group 2 received 50mg/kg Group 3 received 100mg/kg Group 4 received 200mg/kg Crude extract of Cissampelosmucronata orally repeatedly for 21 consecutive days respectively. The animal were scarified after 24 hours after the last day of administration.

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